Patrick Chames' web site

My C.V.

Education
Year	Activity
June 90	Biochemistry degree (2 years post high school) University of Provence , Marseille
June 93	Engineer Diploma at (National Institute of Applied Sciences), Toulouse, Dpt GBAGM (Molecular Biology and Food Engineering). 3rd/36
Sept. 93	Master degree in Molecular Biology and Biotechnology at University paul Sabatier Toulouse (with distinction)
Oct. 94	Scientist for the army at Institute of Tropical Diseases (IMTSSA), Marseille
Oct. 97	Ph. D of Immunotechnology in the group of Dr Daniel Baty, CNRS UPR 9027 (Dr Claude Lazdunski), Marseille (with distinction)
Nov 97 - Jun 01	Four years post doc in the laboratory of Dr Hennie Hoogenboom, Maastricht, NL (EC grant)
Jun 01 - Oct 05	Project leader at Cellectis, the genome engineering company, Paris, FR
Since Oct 05	Scientist for the national research institute CNRS , LISM (UPR 9027) , Daniel Baty's team

Professional background
Year	Activity
1992	Four months stage at GERME, inc (Marseille) on depollution of industrial wastes using 100 l fermentor fixed bacteria.
1993	In vivo study of the expression pattern of two drosophila protein by fusion with bgal and whole mount X-gal staining. (cloning, oocyte injection, Drosophila genetics (HM Bourbon, AM Duprat).
1994	Twelve months on the design of a recombinant vaccine against Yellow Fever Virus (cloning of an envelope protein from RNA and expression in E. coli)
1995-97	Fine tuning of the specificity and affinity of anti-steroid antibodies using directed randomization of paratope residues and phage display, for diagnostic applications
98-2001	Selection of human antibodies against specific class I MHC molecule -peptide complex for diagnostic and therapy of cancer
01-05	Engineering of enconucleases able to induce double strand break in a specific sequence of any genome, to induce site-specific deletions, insertions, mutations by homologous recombination
Since 05	Design, construction and validation of lama antibody based multivalent and/or multispecific molecules as tools for immunotherapy

Oral presentations

Seminar

Production of antibody fragments in E. coli and modification of their specificity. Doctoral school for Life and health sciences, Marseille, April 1996
Optimization of anti-steroid antibodies, laboratory of HR. Hoogenboom, Maastricht, august 1997
Optimization of anti-steroid antibodies , laboratory of G. Winter, Cambridge, Sept. 1997
Engineering the fine specificity of anti-steroid antibodies, Cambridge Antibody Technology (K. Johnson), Cambridge, sept. 1997
Changing anti-steroid specificity, Laboratory of A. Cattaneo, Trieste , oct. 1997
A tumor specific TCR-like antibody, EC meeting, Berlin, December 1998, Maastricht June 1999, Brussels Nov. 1999, Jerusalem May 2000

Invited speaker in international congres

Engineering of anti-steroid antibody paratope. ImmunoAnalysis European Symposium, Basel, august 1997
A tumor specific TCR-like antibody, Tumor immunology meeting, Breukelen, November 1998
Specificity Improvement of an anti-sterođd antibody, IFCC-world lab, Florence, june 1999
A human antibody against a peptide-MHC complex. Phage Club meeting, La Grande Motte, France, May 1999
Plenary lecture: Developing binding ligands using phage display technologies AND Regular lecture: Molecular approaches to discover new tumor targets, Medical applications of modern biotechnology, La habana, Cuba Dec 1999
Selection of human antibodies against the tumor T cell epitope HLA-A1/MAGE-1, 4th Oncolgy symposium, Maastricht, Feb 2000

Congres attented

IBC's 8th conference on Antibody engineering, San Diego, Dec 1997
Phage club meeting, Maastricht, May 1998
European science foundation's workshop on Design of new scaffold units, Brussels, June 1998
Protein Engineering and phage display, Groningen, May 1998
Phage display, application and developments for discovery, Brussels 8-10 sept. 1999
EuroBiochips (DNA and protein arrays), Hamburg, 5-7 june 2000
Biomolecular Diversity, Boston, 11-13 sept 2000
Antibody Engineering, La Jolla, CA, 3-6 dec 2000
Phage Display Technologies, Directed Protein Evolution, Cambridge, MA, 9-10 April 2001

Posters

Cancer Target Identification by Differential Gene Expression Analysis and Phage Antibody Display, Baltimore, June 2000 see abstract
A phage display selected Fab fragment with MHC class I-restricted specificity for MAGE-A1 can retarget primary human T lymphocytes, Dusseldorf, Dec 2000 see abstract

Teaching

1 week training course on phage display technology, Master degree immunotechnology, University of Provence, Marseille, 1997
Co organiser of EMBO course 1999 on phage display (BIAcore measurements), Maastricht, Nov. 1999
Course on of 3D structure visualisation using Rasmol, Maastricht, March 2000

Special distinctions

Special PhD thesis prize of "Universite de la Méditerranée", 1997, Marseille

Civil status

Born in Marseille, France on the 1st October 1970, French nationality, Married, Two children (1999 and 2004)

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